Examination of Thymic Positive and Negative Selection by Flow Cytometry
نویسندگان
چکیده
منابع مشابه
Thymic T-Cell Education: Positive & Negative Selection
TOLERANCE (which is antigen-specific) must be distinguished from immune deficiency (non-specific). Its most important manifestation, the maintenance of SELFTOLERANCE, was originally explained by the Clonal Selection theory as the result of CLONAL ABORTION of self-reactive clones. However, potentially self-reactive Tand B-cells do exist in normal individuals, leading to the recognition of the im...
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Foxp3 expression is a marker of regulatory T cells (Treg), but how early it is expressed in the thymus is still not fully defined. In this study, we examined Foxp3 expression in double-negative (DN) CD4(-)CD8(-) T cell precursors in the thymus by flow cytometry. By increasing the number of collected cells from the conventional 10(4) cells up to more than 10(6) cells during flow cytometry, we fo...
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BACKGROUND Urinary tract infection (UTI) is a widespread infectious disease in humans. Urine culture, a huge workload in the microbiology laboratory, is still the standard diagnostic test for UTI, but most of the cultures are negative. A reliable screening method could reduce unnecessary cultures and quicken reporting of negative results. METHODS We evaluated the usefulness of a flow cytometr...
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Synergy of photoacoustic and fluorescence flow cytometry of circulating cells with negative and positive contrasts.
In vivo photoacoustic (PA) and fluorescence flow cytometry were previously applied separately using pulsed and continuous wave lasers respectively, and positive contrast detection mode only. This paper introduces a real-time integration of both techniques with positive and negative contrast modes using only pulsed lasers. Various applications of this new tool are summarized, including detection...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2012
ISSN: 1940-087X
DOI: 10.3791/4269